Regulatory

Part:BBa_K3431038:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-17)


oz146_B Toehold Switch for miR-146 Detection


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure their minimal free energy are in an appropriate range (lower than 0 kcal/mole)


Source

This is a synthetic sequence based upon Green, A.A. et al. and Pardee, K. et al. for detection of miRNA-31. We obtained this sequence through custom gene synthesis of IDT.

References